Estimation of Paracetamol and Tapentadol in tablet dosage form is routinely carried out but simultaneous estimation of the combination is not available. Hence a simple, rapid, sensitive, economic, and accurate HPLC method has been developed and validated for simultaneous estimation of Paracetamol and Tapentadol in bulk and tablet dosage form. The chromatographic separation was performed in waters e 2695 equipment using mobile phase consisting of OPA and methanol in ratio of 70:30. The column used was Zorbax c18 (150Ã?â??4.6mm ID) 5Ã?µ with flow rate 1 ml/min using PDA detector at 301nm. The RT was found to be 2.954 for paracetamol and 3.651 for Tapentadol. The described method was found to be linear and correlation coefficient was found to be 0.99. The validation of proposed method was carried for its accuracy, precision, and specificity, limit of detection and limit of quantitation for both paracetamol and Tapentadol. The assay values for paracetamol and Tapentadol was found to be 99% and 100%. The results showed that proposed RP-HPLC method is simple precise, reliable, accurate and economical which is useful for routine determination of paracetamol and Tapentadol in bulk drug and its pharmaceutical dosage form.
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